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SEM images of <t>HSFs</t> cultured on PLA scaffolds with varying porosities. The image shows HSF adherence and cell spreading across scaffolds (yellow arrows) with 20%, 40%, 60%, 80%, and 100% porosity. The scaffolds with higher porosity (80% and 100%) demonstrate improved cell adhesion and enhanced spreading, with cells appearing more elongated and distributed uniformly on the surface. In contrast, scaffolds with lower porosity show reduced cell attachment and limited spreading, indicating that higher porosity promotes better cellular interactions with PLA scaffolds.
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SEM images of <t>HSFs</t> cultured on PLA scaffolds with varying porosities. The image shows HSF adherence and cell spreading across scaffolds (yellow arrows) with 20%, 40%, 60%, 80%, and 100% porosity. The scaffolds with higher porosity (80% and 100%) demonstrate improved cell adhesion and enhanced spreading, with cells appearing more elongated and distributed uniformly on the surface. In contrast, scaffolds with lower porosity show reduced cell attachment and limited spreading, indicating that higher porosity promotes better cellular interactions with PLA scaffolds.
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SEM images of <t>HSFs</t> cultured on PLA scaffolds with varying porosities. The image shows HSF adherence and cell spreading across scaffolds (yellow arrows) with 20%, 40%, 60%, 80%, and 100% porosity. The scaffolds with higher porosity (80% and 100%) demonstrate improved cell adhesion and enhanced spreading, with cells appearing more elongated and distributed uniformly on the surface. In contrast, scaffolds with lower porosity show reduced cell attachment and limited spreading, indicating that higher porosity promotes better cellular interactions with PLA scaffolds.
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SEM images of HSFs cultured on PLA scaffolds with varying porosities. The image shows HSF adherence and cell spreading across scaffolds (yellow arrows) with 20%, 40%, 60%, 80%, and 100% porosity. The scaffolds with higher porosity (80% and 100%) demonstrate improved cell adhesion and enhanced spreading, with cells appearing more elongated and distributed uniformly on the surface. In contrast, scaffolds with lower porosity show reduced cell attachment and limited spreading, indicating that higher porosity promotes better cellular interactions with PLA scaffolds.

Journal: Polymers

Article Title: Three-Dimensional Printed Porous PLA Scaffolds with Dual Functionality: Cell Proliferation Enhancement and Antibacterial Properties

doi: 10.3390/polym17141928

Figure Lengend Snippet: SEM images of HSFs cultured on PLA scaffolds with varying porosities. The image shows HSF adherence and cell spreading across scaffolds (yellow arrows) with 20%, 40%, 60%, 80%, and 100% porosity. The scaffolds with higher porosity (80% and 100%) demonstrate improved cell adhesion and enhanced spreading, with cells appearing more elongated and distributed uniformly on the surface. In contrast, scaffolds with lower porosity show reduced cell attachment and limited spreading, indicating that higher porosity promotes better cellular interactions with PLA scaffolds.

Article Snippet: Human Skin Fibroblast (HSFs) (ATCC, Manassas, VI, USA) were cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( v / v ) MEM NEAA (non-essential amino acid), 1% ( v / v ) 200 mM L-glutamine and 2% ( v / v ) antibiotics (6.74 U/mL penicillin-streptomycin) (Sigma-Aldrich, Dorset, UK) in T25 flasks.

Techniques: Cell Culture, Cell Attachment Assay